Hydrogen- Deuterium Exchange Mass Spectrometry (HDX-MS) Services

Hydrogen–deuterium exchange mass spectrometry (HDX-MS) enables the direct
measurement of protein conformational dynamics, stability, and molecular interactions under
near-physiological conditions. Now available at Case Western Reserve University, this
advanced platform combines fully automated HDX workflows with Waters Cyclic Ion Mobility
Mass Spectrometry (cIMS) to provide exceptional structural resolution and confidence.

Applications:

 Epitope mapping
 Protein–ligand and protein–protein interaction mapping
 Enzyme and signaling protein dynamics
 Structural effects of mutations and post-translational modifications
 Study of protein misfolding and pathological protein conformations

Waters Cyclic Ion Mobility Mass Spectrometry (cIMS) System coupled with a Trajan HDX
Workstation, supported by an NIH Shared Instrumentation Grant (S10OD036418-01A1)